Pharmacological properties of YM461, a new orally active platelet-activating factor antagonist |
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Authors: | Toshimitsu Yamada Munetoshi Saito Toshiyasu Mase Hiromu Hara Hitoshi Nagaoka Kiyoshi Murase Kenichi Tomioka |
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Affiliation: | (1) Medicinal Research Laboratories, Yamanouchi Pharmaceutical Co., Ltd., 21 Miyukigaoka, 305 Tsukuba, Ibaraki, Japan |
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Abstract: | The antagonistic effect of YM461 1-(3-phenylpropyl)-4-2-(3-pyridyl)thiazolidin-4-ylcarbonyl]piperazine fumarate] against
platelet-activating factor (PAF) was examined in severalin vitro andin vivo systems. We found that YM461 inhibited 3H]PAF binding to rabbit platelet membranes with a pKi value of 8.90. YM461 inhibited PAF induced rabbit and human platelet aggregation with pA2 values of 7.52 and 7.29, respectively; the slopes of the Schild plots were 1.07 and 1.01, respectively. However, YM461 at
10−4M did not affect rabbit and human platelet aggregation induced by ADP, collagen, arachidonic acid or epinephrine. YM461 inhibited
PAF induced death in mice with an ED50 (50% effective dose) value of 0.35 mg/kgp.o. YM461 at doses above 0.3 mg/kgi.v. inhibited PAF induced hypotension in rats. YM461 showed a dose-dependent inhibition of PAF induced hemoconcentration in rats
with ED50 values of 0.15 and 0.21 mg/kgp.o., respectively, at 0.5 and 1 hr after oral administration. The anti-PAF effect of YM461 persisted more than 6 hr after 3 mg/kgp.o. in rats. YM461 inhibited the bronchoconstriction induced by PAF with an ED50 value of 1.2 mg/kgp.o. in anesthetized guinea pigs. Furthermore, the compound at doses above 3 mg/kgp.o. significantly inhibited antigen-induced anaphylactic asthma in conscious guinea pigs pretreated with mepyramine and propranolol.
These results indicate that YM461 is a selective, potent and orally active PAF antagonist.
Based on a paper presented at the Third International Conference on Platelet-Activating Factor and Structurally Related Alkyl
Ether Lipids, Tokyo, Japan, May 1989. |
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