猪血红蛋白的酶解工艺研究

目的 研究猪血红蛋白的酶解工艺.方法 用组合酶Ⅰ（蛋白酶F与蛋白酶P）和组合酶Ⅱ（蛋白酶F与蛋白酶N）同步酶解猪血红蛋白.结果 确定最佳工艺参数,组合酶Ⅰ的最佳酶解条件为：酶添加量0.2％,温度50℃,料水比1∶1.25,pH 7.5,水解时间20 h;组合酶Ⅱ的最佳酶解条件为：酶添加量0.24％,温度50℃,料水比1：1.75,pH 8.0,酶解时间20h.此工艺条件下,组合酶Ⅰ和组合酶Ⅱ三氯乙酸可溶性氮含量（TCA-SN指数）分别为71.72％和87.82％,两种组合酶的蛋白质回收率均达90％.结论 组合酶Ⅱ较适于工业生产.

Study on Enzymolysis Technology of Porcine Hemoglobin

Objective To study the enzymolysis technology of porcine hemoglobin.Methods Porcine hemoglobin was synchronously hydrolyzed by the mixed enzyme Ⅰ （Protease F and Protease P） and the mixed enzyme Ⅱ （Protease F and Protease N）.Results The optimum conditions for the mixed enzyme Ⅰ were as follows：enzymatic addition 0.2 ％,enzymolysis temperature 50 ℃,ratio of material to water of 1∶1.25,pH 7.5,enzymolysis time 20 hours.The optimum conditions for the mixed enzyme Ⅱ were as follows：enzymatic addition 0.24 ％,enzymolysis temperature 50 ℃,ratio of material to water of 1：1.75,pH 8.0,enzymolysis time 20 hours.Under those conditions,the protein recovery rate of two kinds of the mixed enzyme both reached 90％ and the trichloroacetic acid-soluble nitrogen （TCA-SN） contents were 71.72 ％ and 87.82 ％,respectively.Conclusion The mixed enzyme Ⅱ can be suitable for the industrial production.