| HPLC测定米力农注射液中的乳酸含量 |
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| 引用本文： | 刘永久,姚作进,徐玉文. HPLC测定米力农注射液中的乳酸含量[J]. 山东食品科技, 2013, 0(6): 416-418 |
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| 作者姓名： | 刘永久 姚作进 徐玉文 |
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| 作者单位： | [1]淄博市中心医院,山东淄博255000 [2]山东省兖矿鲁南化肥厂职工医院,山东滕州277527 [3]山东省食品药品检验所,山东济南250101 |
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| 基金项目： | 国家药典委员会资助（国药典化发[2010]84号） |
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| 摘 要： | 目的 建立测定米力农注射液中乳酸含量的HPLC方法.方法 采用Agilent SB-Aq （4.6 mm×250 mm,5μm）色谱柱,甲酸-二环己铵-水（0.1：0.1：100）为流动相,流速：1mL/min,检测波长210nm,柱温：25℃,进样量：20μL.结果 乳酸在0.5～5.0mg/mL的浓度范围内与峰面积线性关系良好（r=0.9999）,平均回收率为99.7％（RSD=0.37％,n=6）.结论 此法准确、简便、快速,适用于米力农注射液中乳酸的含量测定.
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| 关 键 词： | 米力农注射液 乳酸 含量测定 高效液相色谱法 |
Determination of Lactate in Milrinone Injection by HPLC |
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| LIU Yong-jiu,YAO Zuo-jin,XU Yu-wen. Determination of Lactate in Milrinone Injection by HPLC[J]. , 2013, 0(6): 416-418 |
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| Authors: | LIU Yong-jiu YAO Zuo-jin XU Yu-wen |
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| Affiliation: | 1.Zibo Center Hospital, Zibo 255000, China; 2.Lunan Yankuang Chemical Fertilizer Plant Worker＇s Hospital,Tengzhou 277527, China; 3.Shandong Provincial Institute for Food and Drug Control, Jinan 250101, China) |
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| Abstract: | Objective To establish a HPLC method for the determination of lactate in milrinone injection.Methods An Agilent SB-Aq column （250 mm× 4.6 mm,5 μm） was used with formic acid-dicyclohexylamine-water （0.1：0.1：100） as the mobile phase.The flow rate was 1.0 mL/min and the detection wavelength was 210 nm.The temperature of the column was 30 ℃ and the sample amount was 10 μL.Results There was a good linear relationship between the concentration of lactate within 0.5-5.0 mg/mL and the peak area （r=0.999 9）.The average recovery rate was 99.7 ％ （RSD=0.37 ％,n=6）.Conclusion This method can be accurate,simple,quick and suitable for the determination of lactate in milrinone injection. |
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| Keywords: | milrinone injection lactate content determination HPLC |
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