超声波萃取黄秋葵籽油的脂肪酸组成及其抗氧化能力评价

以黄秋葵种子为原料,正己烷为提取溶剂,采用超声波提取法提取黄秋葵种子中的籽油,气相色谱分析其提取物脂肪酸的种类和相对含量,并通过ABTS~+·自由基清除试验、DPPH自由基清除试验评价籽油的体外抗氧化能力,碘量滴定法测定过氧化值。结果表明,黄秋葵籽油中主要含有13种脂肪酸,以亚油酸(42.05%)、棕榈酸(26.43%)、油酸(20.58%)、十一烷酸(5.1%)、硬脂酸(3.4%,)为主,其过氧化值为5.86mmol/kg。体外抗氧化研究表明,黄秋葵籽油对DPPH自由基清除能力当量于0.5 mgVC/mL的清除能力,对ABTS~+·清除能力当量于285 mg Trolox/mL的清除能力。

The Fatty Acid Composition and Antioxidation Activities of Okra Seed Oil

The okra seeds oil was obtained by the ultrasonic extraction method with hexane as the extraction solvent. The composition and relative content were also analyzed by the gas chromatographic. The antioxidant capacity in vitro was evaluated by the ABTS + ?and DPPH radical scavenging experiments. In addition, the peroxide value of the sample was determined by the iodometric titration. The results showed that okra seed oil mainly contained 13 kinds of fatty acids including linoleic acid (42.05%), palmitic acid (26.43%), oleic (20.58%), undecanoate (5.1%), stearic acid (3.4%) and the peroxide value was 5.86 mmol/kg. The results of antioxidant activity in vitro indicated that the DPPH radical scavenging capacity of okra seeds oil was equal to 0.5 mg VC/mL and the ABTS ?scavenging capacity was equal to 285 mg Trolox /mL.