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Quantum Dots as Fluorescent Labels for Detection of Heat Shock Protein in Tumor Tissue Using Laser Scanning Confocal Microscope |
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| Authors: | YANG Huan YE Zhangqun CHEN Zhiqiang ZHANG Jie YE Peng |
| Affiliation: | YANG Huan1,YE Zhangqun1,CHEN Zhiqiang1,ZHANG Jie2,YE Peng3 (1. Department of Urology,Tongji Hospital,Tongji Medical College,Huazhong University of Science , Technology,Wuhan 430030,China,2. Department of Urology,Renmin hospital of Wuhan University,Wuhan 430060,3. Department of Pathology,China) |
| Abstract: | A new Quantum Dots(Qdots) nanocrystal composed of semiconductor core and zinc sulfide shell, and its feasibility as labels in immunofluorescence analysis for the imaging of tumor biomarkers by laser scanning confocal microscope(LSCM) was investigated. Qdots taged by mercaptoacetic acid were conjugated with second antibody, then imaging differences of Heat Shock Proteins 70(HSP70) in renal carcinoma tissure sections with immunofluorescence analysis method using Qdots bioconjugates and conventional organic dy... |
| Keywords: | quantum dots immunofluorescence analysis bioimaging laser scanning confocal microscope |
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