Trennung von Triglyceriden nach Kettenlänge und Sättigungsgrad durch HPLC

Separation of Triglycerides According to Chain Length and Degree of Saturation by HPLC In routine fat analysis HPLC is efficiently used. For constant work columns with chemically bound C₁₈-groups are especially suitable. There are critical pairs of fatty acid-methylesters which however can be separated by means of elution agents basing on acetonitril. Oleic acid and elaidic acid can be separated by this way, too. By GLC triglycerides can only be separated according to the number ob C-atoms, whereas in HPLC double bounds have a great influence on the retention time, too. This is especially evident if elution agents, basing on nitrils, are used. The separation principle was determined by capillar gas chromatographic analysis of the HPLC fractions. Further on hints are given to avoid technical difficulties in the use of differential refractometers.