Fluorometric detection of lectin with water-soluble hyperbranched conjugated polymer using mannose mediation |
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Authors: | Kim Daigeun Jang Geun Seok Son Ji Hye Seo Seongwon Kim Jongho Kim Tae Hyeon Lee Taek Seung |
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Affiliation: | Organic and Optoelectronic Materials Laboratory, Department of Nanotechnology, Chungnam National University, Daejeon 305-764, Korea. |
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Abstract: | A water-soluble hyperbranched polymer containing boronic acid groups at the ends of the polymer, which are capable of binding to diol-containing mannose, was syntheized. The hyperbranched polymer was prepared by a palladium-catalyzed Suzuki cross-coupling reaction using the tribromo monomer for the hyperbranched type structure. The water-soluble hyperbranched polymer (HP) exhibited enhanced fluorescence intensity upon exposure to lectin in the presence of mannose compared to other proteins, such as lysozyme and cytochrome c, because mannose plays a key role in binding both lectin and HP resulting in selective sensing toward lectin. |
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