固相萃取-高效液相色谱-串联质谱法测定食品中的丙烯酰胺

建立高效液相色谱-串联质谱仪测定食品中丙烯酰胺含量的方法。以D3]-丙烯酰胺为同位素内标，使用20mg/mL淀粉酶溶液对样品进行酶解，经水浴、除脂、离心，利用固相萃取柱进行净化并浓缩后，通过高效液相色谱-串联质谱仪以多选择反应监测模式进行分析测定。该方法的检出限和定量限分别为4.8μg/kg和16.5μg/kg。本方法具有操作方便、准确率高、重复性好等优点，可广泛应用于各类食品中丙烯酰胺含量的检测。

Determination of Acrylamide in Foods by Solid-phase Extraction and HPLC-MS/MS

A method using D3]-acrylamide as an isotope internal standard for determining acrylamide in foods was established by solid-phase extraction coupled with high performance liquid chromatography and quadrupole tandem mass spectrometry(HPLC-MS/MS).Analytical procedures were composed of amylase hydrolysis at a concentration of 20 mg/mL,the removal of fat,cleanup by solid-phase extraction(SPE) and the determination by HPLC-MS/MS with a multi-reaction monitoring(MRM) mode and the addition of an internal standard.The limit of detection(LOD) and limit of quantitation(LOQ) of the developed method were 4.8 and 16.5μg/kg,respectively.This method has the advantages of convenient operation,high sensitivity,excellent accuracy and good reproducibility and is therefore suitable for the determination of acrylamide in foods.