Inactivation of Listeria innocua in brined white cheese by a combination of nisin and heat |
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Authors: | Murad A Al-Holy Anas Al-NabulsiTareq M Osaili Mutamed M AyyashReyad R Shaker |
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Affiliation: | a Department of Clinical Nutrition and Dietetics, Faculty of Allied Health Sciences, Hashemite University, P.O Box 150459, Zarqa 13115, Jordan b Department of Nutrition and Food Technology, Faculty of Agriculture, Jordan University of Science and Technology, P.O. Box 3030, Irbid 22110, Jordan c School of Biomedical and Health Sciences, Faculty of Health, Engineering and Science, Victoria University - Werribee Campus, P.O. Box 14428, Victoria 8001, Australia |
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Abstract: | The objective of this study was to investigate the effect of nisin alone and in combination with heat (63 °C/5 min) on the inactivation of Listeria innocua in white cheese. Nisin was added at different concentrations (500, 1000, and 1500 IU ml−1) to pasteurized milk before curd formation. The curd was soaked for 24 h in 10% solution of brine containing ca 106 CFU ml−1 of a cocktail mixture of three strains of L. innocua. Part of the nisin treated samples were heat treated at 63 °C/5 min. Total mesophilic count (TMC), L. innocua survivors and changes in the pH of white cheese were monitored each 2 d for a period of 12 d of storage at 4 or 10 °C. Nisin at 500 IU ml−1 did not diminish TMC in white cheese compared to the control. The combination of heat and nisin (1000 or 1500 IU ml−1) exhibited a bacteriostatic effect on TMC throughout the storage period at 4 or 10 °C. Nisin at 500 IU ml−1 had a marginal inhibitory activity against L. innocua. However, nisin at 1000 and 1500 IU ml−1l resulted in a more than 2 log10 reduction in L. innocua count and the effect was more prominent at 10 °C. In comparison, the combination of nisin (1000 or1500 IU ml−1) and heat treatment exhibited a synergistic inhibitory activity against L. innocua, where a complete elimination of the organism was accrued after 6 and 8 d of storage at 10 and 4 °C. Therefore, nisin and heat combination could be used as a prudent hurdle to preclude the growth of Listeria in white cheese, especially under the condition of abused refrigeration conditions. |
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Keywords: | Listeria innocua White cheese Nisin Heat Synergy |
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