大蒜粉功效成分检测方法的优化

目的通过对比美国药典,欧洲药典和中国药典检测大蒜(粉)功效成分的区别,理清国内外大蒜(粉)功效成分的研究方向,并对美国药典检测大蒜粉的功效成分——蒜氨酸的检测方法进行优化,提出一个操作更加简便,计算更加准确的蒜氨酸检测方法。方法称取试样适量用70%乙醇超声提取,进行高效液相色谱法分析,用十八烷基硅烷键合硅胶为填料的色谱柱分离,流动相为甲醇:5 mmol/L庚烷磺酸钠(磷酸调p H至2),蒜氨酸标准品(美国药典)定量计算其含量。结论蒜氨酸浓度在0.035616~0.213696 mg/m L的范围内与峰面积的线性良好,相关系数r0.999。在80%、100%、120%添加水平下,蒜氨酸的回收率为98.0%。结论该方法操作简便、准确、重现性好,适用于大蒜粉中蒜氨酸的含量测定。

Optimization of detection method for garlic powder composition

Objective By comparing the difference of garlic (powder) efficacy composition in the United States Pharmacopoeia, European Pharmacopoeia and Chinese Pharmacopoeia, the optimization method of alliin was researched. Considering the detection of garlic powder composition in the United States Pharmacopoeia, the paper proposed a more simple operation with more accurate calculation for alliin detection. Methods The sample was extracted with 70% ethanol ultrasonic and then analyzed by high performance liquid chromatography (HPLC), and octadecyl silane bonded silica gel as the filler of chromatographic column separation was used. The mobile phase of methanol: 5 mmol/L sodium heptane sulfonate (the pH was adjusted by phosphate to 2), the content was calculated by alliin standard (USP) quantitatively. Results Alliin concentration in 0.035616~0.213696 mg/mL had a good linearity with peak area, and within the range the correlation coefficients r>0.999. At the level of adding at 80%, 100%, and 120%, the recovery rate was 98.0%. Conclusion The method is simple, accurate and reproducible, and suitable for determination of the alliin content in garlic powder.