Identification of a novel actin binding site within the Dp71 dystrophin isoform

A method is described by which the diffusion coefficients of electrically charged micelles can be determined using micellar electrokinetic chromatography (MEKC). The determination is based on a theory for the dispersion, at low electric field strengths, of analytes that are solubilized by only the micellar phase. The dispersion is represented by contributions from instrumental sources and from longitudinal diffusion. The latter depends on the micellar diffusion coefficient. The theory is used to determine the micellar diffusion coefficients of the surfactant sodium dodecyl sulfate in three solutions having different surfactant concentrations. These diffusion coefficients compare very favorably with those determined by diffusion-ordered two-dimensional NMR spectroscopy. An argument is presented justifying that the diffusion coefficients determined by MEKC are self-diffusion coefficients.