Development of a microfluidic platform with an optical imaging microarray capable of attomolar target DNA detection |
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Authors: | Bowden Michaela Song Linan Walt David R |
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Affiliation: | Department of Chemistry, Tufts University, Medford, Massachusetts 02155, USA. |
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Abstract: | In this paper, DNA hybridization in a microfluidic manifold is performed using fluorescence detection on a fiber-optic microarray. The microfluidic device integrates optics, sample transport, and fluidic interconnects on a single platform. A high-density optical imaging fiber array containing oligonucleotide-labeled microspheres was developed. DNA hybridization was observed at concentrations as low as 10 aM with response times of less than 15 min at a flow rate of 1 microL/min using 50 microL of target DNA samples. The fast response times coupled with the low sample volumes and the use of a high-density, fiber-optic microarray format make this method highly advantageous. This paper describes the initial development, optimization, and integration of the microfluidic platform with imaging fiber arrays. |
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