The triacylglycerol structure of olive oil determined by silver ion high-performance liquid chromatography in combination with stereospecific analysis |
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Authors: | F Santinelli P Damiani W W Christie |
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Affiliation: | (1) Present address: Istituto di Chimica Bromatologica, Universita degli Studi, 06100 Perugia, Italy;(2) Hannah Research Institute, KA6 5HL Ayr, Scotland |
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Abstract: | The compositions of positionssn-1,sn-2 andsn-3 of triacylglycerols from “extra-virgin” olive oil (Olea europaea) were determined. The procedure involved preparation of diacyl-rac-glycerols by partial hydrolysis with ethyl magnesium bromide; 1,3-, 1,2- and 2,3-diacyl-sn-glycerols as (S)-(+)-1-(1-naphthyl)ethyl urethanes were isolated by highperformance liquid chromatography (HPLC) on silica, and their fatty
acid compositions were determined. The same procedure was also carried out on the five main triacylglycerol fractions of olive
oil after separation according to the degree of unsaturation by HPLC in the silver ion mode. Although stereospecific analysis
of the intact triacyl-sn-glycerols indicated that the compositions of positionssn-1 andsn-3 were similar, the analyses of the molecular species demonstrated marked asymmetry. The data indicate that the “1-random,
2-random, 3-random” distribution theory is not always applicable to vegetable oils. |
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Keywords: | Chiral chromatography diacylglycerols high-performance liquid olive oil (Olea europaea) silver ion chromatography stereospecific analysis triacylglycerols |
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