Comparison of structural,antioxidant and immuno‐stimulating activities of polysaccharides from Tremella fuciformis in two different regions of China |
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| Authors: | Pan Li Zhuo Jiang Tian Sun Chao Wang Yiyong Chen Ziyin Yang Bing Du Chunyang Liu |
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| Affiliation: | 1. College of Food Science, South China Agricultural University, Guangzhou, China;2. Infinitus (China) Company Ltd, Guangzhou, China;3. Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China |
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| Abstract: | In this study, we compared the chemical structure, antioxidant and immuno‐stimulating activities of two polysaccharides, TPS–FJ and TPS–SC, extracted from Tremella fuciformis from two of the major cultivation regions of China. TPS–FJ and TPS–SC contained protein and high uronic acid. Average molecular weights of TPS–FJ and TPS–SC were 45 461 and 25 981 kDa, respectively. A triple‐helix conformation was exhibited by TPS–FJ but not by TPS–SC. TPS–FJ and TPS–SC contained same monosaccharides but in different ratios. Both the polysaccharides displayed characteristic polysaccharide bands in their Fourier transform infrared and NMR spectra, shown high water and oil holding capacity, and retained antioxidant potential after simulated gastrointestinal digestion in vitro. TPS–FJ significantly (P < 0.05) stimulated the nitric oxide, tumour necrosis factor‐α and interleukin‐6 production of RAW 264.7 cells with higher reactions than those of TPS–SC in vitro. Thus, Tremella fuciformis polysaccharide can be used as a potential antioxidant and immunomodulatory ingredient in food industry. |
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| Keywords: | Gastrointestinal digestion immuno‐stimulating activity polysaccharide structural
Tremella fuciformis
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