| 响应面法优化毛云芝菌产漆酶的培养条件研究 |
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| 引用本文： | 范文霞,蔡友华,刘学铭,肖更生,陈卫东. 响应面法优化毛云芝菌产漆酶的培养条件研究[J]. 现代食品科技, 2008, 24(2): 153-156 |
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| 作者姓名： | 范文霞 蔡友华 刘学铭 肖更生 陈卫东 |
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| 作者单位： | 1. 广东省农业科学院蚕业与农产品加工研究所,广东省农产品加工公共实验室,广东,广州,510610;江西农业大学生物科学与工程学院,江西,南昌,330045
2. 广东省农业科学院蚕业与农产品加工研究所,广东省农产品加工公共实验室,广东,广州,510610 |
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| 基金项目： | 教育部留学回国人员科研启动基金 |
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| 摘 要： | 研究了影响毛云芝菌产漆酶的培养条件,通过单因素实验选取试验各因素的水平,根据Box-Benhnken试验设计原理,进行响应面回归分析,以漆酶酶活为响应值,确定了影响漆酶产量的各因素的水平,结合验证实验结果得到其最佳的培养条件为接种量8.2 %、装液量30.8%、摇床转速141 r/min.
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| 关 键 词： | 响应面法 毛云芝菌 漆酶 培养条件 |
| 文章编号： | 1673-9078(2008)02-0153-04 |
| 收稿时间： | 2007-11-06 |
| 修稿时间： | 2007-11-06 |
Optimization of Culture Conditions of a White-Rot Fungus for Laccase Production by Response Surface Design |
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| FAN Wen-xi,CAI You-hu,LIU Xue-ming,XIAO Geng-sheng,CHEN Wei-dong. Optimization of Culture Conditions of a White-Rot Fungus for Laccase Production by Response Surface Design[J]. Modern Food Science & Technology, 2008, 24(2): 153-156 |
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| Authors: | FAN Wen-xi CAI You-hu LIU Xue-ming XIAO Geng-sheng CHEN Wei-dong |
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| Affiliation: | (1.Sericulture & Farm Produce Processing Research Institute of Guangdong Academy of Agricultural Sciences , Guangdong Open Access Laboratory of Agricultural Product Processing, Guangzhou 510610, China)(2.College of Bioscience & Bioengineering , Jiangxi Agricultural University, Nanchang 330045, China) |
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| Abstract: | The fermentation conditions of a white-rot fungus Coriolus hirsutus for laccase production were optimized by single factor experiment and response surface design. The results showed that the optimal inoculum size, amount of broth in flask and shaking rate were 8.2%, 30.8% and 141r/min, respectively. |
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| Keywords: | response surface design Coriolus hirsutus laccase fermentation condition |
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