固相微萃取/气相色谱-质谱法分析小茴香挥发性成分

考察了萃取样品温度、萃取纤维吸附时间及解吸时间对于固相微萃取的影响,确定了固相微萃取小茴香挥发性成分较佳的实验条件为:萃取样品温度60℃,萃取纤维吸附时间40min,脱附温度250℃,脱附时间5min。用计算机谱库检索结合气相色谱保留指数二维定性方法,从小茴香的固相微萃取/气相色谱-质谱联用分析谱图中,鉴定出反式-茴香脑、顺式-茴香脑、爱草脑、葑酮、p-茴香醛、1,8-桉树脑、樟脑等35种小茴香挥发性成分,总质量分数98·70%。

Analysis of the Volatile Constituents from Foeniculum Vulgare Mill by Gas Chromatography-Mass Spectrometry Coupled with Solid Phase Microextraction

Factors of sample temperature,adsorption time and desorption time on the SPME(solid phase microextraction) treatment of seeds of Foeniculum vulgare Mill were investigated.The optimal extraction conditions found were sampling at 60 ℃ for 40 min and desorption at 250 ℃ for 5 min.Samples of seeds of Foeniculum vulgare Mill were analyzed by GC-MS combining with SPME.Through comparison with mass spectra database and their retention indices relative to n-alkanes,thirty-five components were identified,covering 98.70% of the total peak areas in the GC-MS total ion current chromatography.The characteristic components were trans-anethole,cis-anethole,estragole,fenchone,p-anisaldehyde,eucalyptol and camphor.The present work was useful for the speedy analysis of Foeniculum vulgare Mill and further understanding of its constitution and its pharmacology.