Study of the metabolism of spiramycin in pig liver |
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Authors: | P Mourier A Brun |
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Affiliation: | Rh?ne Poulenc Rorer, Centre de Recherche de Vitry Alfortville, Vitry sur Seine, France. |
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Abstract: | A major metabolic pathway of spiramycins in pig liver is described. This biochemical reaction involves L-cysteine--a common amino acid present in most animal tissues--which reacts with the aldehyde function of the antibiotic forming a thiazolidine ring. This transformation of spiramycin derivatives drastically increased their polarity. A preliminary HPLC method enabling the quantitation of each metabolite in the range 0.5 microg/g of liver tissue is proposed. Spiramycin S is used as an internal standard while extraction procedures take into account the physico-chemical properties of the thiazolidine moieties. By comparison, previous HPLC methods underestimated the exact amount of antibiotic residues because these metabolites were not extracted from the studied tissues. |
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