Sugar and Cyclitol Determination in Vegetables by HPLC Using Postcolumn Fluorescent Derivatization |
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| Authors: | RYOICHI MASUDA KATSUYOSHI KANEKO ICHIJI YAMASHITA |
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| Affiliation: | Authors Masuda and Kaneko are with the National Food Research Institute, 2-1-2 Kannondai, Tsukuba, 305 Japan. Author Yamashita's present address: National Research Institute of Vegetables, Ornamental Plants and Tea, Anou, Mie, 514-01 Japan. |
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| Abstract: | Nonreducing sugars such as sucrose, raffinose, myo-inositol, pinitol and galactinol were separated on a polyamine-bonded polymer gel (NH2 type) column using acetonitrile-water as mobile phase. Sugars in the eluate were detected by periodate oxidation and the subsequent derivation of guanidine to fluorescent compounds. Detection limits were: sucrose 2.5, D-glucose 2.5 and pinitol 4 ng. This highly sensitive method was more accurate and rapid for determining sugar contents in immature soybean seeds, sweet corn kernels and chinese mustard leaves than the conventional method using refractive index detection. Also, this method reduced the injection volumes of extracts to the column and allowed to lengthen the column life. |
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| Keywords: | soybean corn sugars cyclitols HPLC |
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