7-氨基-4-甲基香豆素分子印迹纳米球的制备与研究

采用单步溶胀法,在单分散纳米级聚苯乙烯微球为种球的基础上,以α-甲基丙烯酸为功能单体,二甲基丙烯酸乙二醇酯为交联剂,乙腈作为制孔剂,7-氨基-4-甲基香豆素(AMC)作为模板分子,成功制得了多孔单分散分子印迹聚合物。通过扫描电镜、紫外光谱和红外光谱等手段对聚合物进行了表征,研究表明,所制得的MIPs平均粒径为200~300 nm,表面粗糙,具有一定孔径,特异吸附性能良好。分析显示,MIPs在识别6-甲基香豆素的过程中存在两类结合位点,高亲和力结合位点的平衡离解常数KD1=49.019μmol·L-1,Bmax1=29.162μmol·g-1;低亲和力结合位点的KD2=84.034μmol·L-1,Bmax2=40.017μmol·g-1。

The Preparation and Study of 7-Amino-4-Methyl Cumarin Molecular Imprinting Nanospheres

On the basis of nanometer-sized polystyrene（PS） microspheres, The monodisperse MIPs were successfully prepared using methacrylic acid as functional monomer, ethylene glycol dimethacrylate as crosslinker, acetonitrile as porogen and 7-amino-4-methy lcumarin （AMC）as the template molecule by a single-srep swelling and polymerization method. The polymer is characterized by several method with scanning electron microscope, ultraviolet spectrum and infrared spectrum. The research shows that the MIPs, rough and porous in surface, has a excellent specific sorption, while its average particle diameter is 200-300 nm. The Equilibrium dissociation constant Kol and maxinum number Bmaxl of binding site with high affinity were calculated to be 49.019 pmol.L-1 and 29.162 mol＇g i, respectively. While KD2 and Bm2 of bingding site with iow affinity were 84.034mol＇L-1 and 40.017molg-1.