高效液相色谱法测定化妆品中的8种抗氧化剂

采用反相高效液相色谱法分别对化妆品样品中可能含有的没食子酸丙酯（PG）、叔丁基对羟基茴香醚（BHA）、2，6-二叔丁基对甲酚（BHT）、没食子酸辛酯（OG）、没食子酸十二酯（DG）、正二氢愈创酸（NDGA）、叔丁基对苯二酚（TBHQ）、2，6-二叔丁基-4-甲氧基苯酚（Ionox-100）等8种抗氧化剂进行检测，色谱条件：色谱柱 Waters Sunfire（150 mm×3．9 mm×5μm），流动相A为水（含质量分数0．5％甲酸），B 为乙腈（含质量分数0．5％甲酸），梯度洗脱，采用紫外检测器，检测波长280 nm，柱温30℃。结果发现，在0．1～10μg/mL 范围内，这8种抗氧化剂浓度与峰面积之间的线性关系良好，相关系数为0．9990～0．9998，检出限为0．05μg/mL。反相高效液相色谱法具有分析速度快、检测灵敏度高、成本低等特点，适用于对化妆品的快速定性定量检测。

Determination of Eight Antioxidants in Cosmetics by Reversed-phase High Performance Liquid Chroma-tography

To develop an reversed-phase HPLC method for determination of propyl gallate（PG）,butylated hydroxyanisole （BHA）,butylated hydroxytoluene（BHT）,n-octyl gallate（OG）,dodecyl gallate（DG）,nordihydroguaiaretic acid （NDGA）, tert-butylhydroquinone（TBHQ）and 2,6-ditertbutyl-4-hydroxy-methylphenol（Ionox-100）in cosmetics. Chromatographic con-ditions included an Waters Sunfire （150 mm×3.9 mm×5 μm）column with a mobile phase of A （water,with 0.5% formic acid by weight）and B （acetonitrile,with 0.5% formic acid by weight）,UV detector and detection wave length 280 nm,col-umn temperature maintained at 30 ℃ and flow rate of 0.6 mL/min. The standard curve of 8 antioxidants was linear in the range of 0.1 to 10μg/mL,with coefficients of 0.9990 to 0.9998.The method was validated in terms of linearity（r〉0.999）. This method has advantages of quick,high sensitivity and low cost,and it is applicable to the rapid qualitative and quantitative de-tection for cosmetics.