高效液相色谱法测定高纯度邻羟基苯甲醚

对用高效液相色谱-反相色谱内标法测定高纯度邻羟基苯甲醚进行了研究。采用C18反相柱，流动相为V（甲醇）：V（质量分数为0．2％乙酸水溶液）=50：50，紫外检测波长为275nm，流速为0．8mL／min。邻羟基苯甲醚的最低检测质量浓度为0．04ug／mL，在0．1～100ug／mL范围内邻羟基苯甲醚的峰面积与质量浓度的线性关系良好（y=0．9999）。以对苯二酚为内标物，用内标法检测高纯度邻羟基苯甲醚含量。用此方法邻羟基苯甲醚回收率为99．9％～100．2％。

Determination of Pure 2-Methoxyphenol by High-performance Liquid Chromatography

A reversed-phase high-performance liquid chromatographic method is used for the determination of pure 2-methoxyphenol. The conditions are as follows： a C18 column, the mobile phase V（methanol） ： V（0.2% acetic acid） = 50：50, 2-methoxyphenol monitored at 275 nm by UV detector, the flow rate 0.8 mL/min, and the detection limit 0. 04 ug/mL. A good liner correlation was found between the peak areas and the concentrations in the range of 0.1- 100 ug/mL of 2-methoxyphenol （y=0. 999 9）. The ranges of the recovery of 2-methoxyphenol were 99.9% -100.2% with hydroquinone as internal standard.