云烟净油的制备及主要成分

为脱除烟草超临界提取物中的蜡质成分，降低其在卷烟中使用时产生的不良风味，以云南清香型烟叶模块碎片为原料，通过超临界CO₂提取、乙醇脱蜡和冷冻离心等工艺，制得云烟净油及其蜡质脱除物；并通过GC-MS分析和感官质量评价，确定了各组分的化学组成及感官作用。结果表明:①该工艺的蜡质脱除率高达99.83%，21种C₂₅~C₃₃的蜡质烷烃得到有效脱除；②烟草超临界提取物经脱蜡后可有效改善卷烟感官作用；③超临界提取物的脱蜡工艺可实现烟草致香成分、烟草甾醇和天然维生素E的有效富集。该乙醇脱蜡方法可用于烟草超临界提取物的精制化。 

Absolute oil of Yunyan tobacco: preparation and major components

In order to remove the waxy portions in supercritically extracted tobacco and to reduce unpleasant flavoring characters of the extracts, Yunyan tobacco absolute oil and its dewaxed product were prepared from Yunnan tobacco leaves with fresh flavor. The waxy components were removed by supercritical CO₂ extraction, dewaxing with alcohol and refrigerated centrifugation. The chemical compositions and sensory properties of each dewaxed fraction were determined by GC-MS analysis and sensory quality evaluation. The results showed that: 1) The efficiency of the dewaxing process was as high as 99.83%. Twenty-one alkanes of C₂₅-C₃₃ in tobacco were effectively removed. 2) The sensory properties of the treated extract were significantly improved after dewaxing. 3) The dewaxing process effectively enriched tobacco aroma components, tobacco sterols and natural vitamin E. The dewaxing process with alcohol was considered suitable for the purification of supercritically extracted tobacco.