| 中华草龟肉抗肿瘤活性肽的分离纯化及鉴定研究 |
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| 引用本文： | 石扬,张永进,赖年悦,林琳,姜绍通,陆剑锋. 中华草龟肉抗肿瘤活性肽的分离纯化及鉴定研究[J]. 现代食品科技, 2018, 34(5): 24-31 |
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| 作者姓名： | 石扬 张永进 赖年悦 林琳 姜绍通 陆剑锋 |
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| 作者单位： | 合肥工业大学食品科学与工程学院;合肥市畜牧水产技术推广中心 |
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| 基金项目： | 安徽省水产产业技术体系项目（皖农科[2016]84号） |
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| 摘 要： | 该研究选取中华草龟肉为原料,以其木瓜蛋白酶酶解所得多肽对人乳腺癌细胞MCF-7增殖的抑制功效为活性检测指标,采用MTT细胞毒性实验对具有抗癌作用的多肽组分进行追踪测定。先采用截留分子量10 ku的超滤膜对酶解液进行截留,再通过Sephadex G-75、G-50及LH-60等层析技术手段对截留所得活性成分进行分离纯化。RP-HPLC谱图的结果表明,最终分离纯化出了一个纯度较高的抗癌活性组分TP-1,其IC50值约为2.7 mg/mL,呈明显的抗癌剂量依赖性与时间依赖性,且对正常细胞毒性极小。随后通过紫外、红外、圆二色谱对TP-1进行理化性质表征,并采用质谱技术对该多肽主成分分子量进行鉴定。结果表明TP-1中含有β折叠,β转角和无规则卷曲结构,且主成分多肽分子量约为1410.7 u,本研究成果为进一步开展中华草龟活性肽的抗癌机理研究奠定了坚实基础。
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| 关 键 词： | 中华草龟；抗肿瘤；抗癌；生物活性肽；分离；纯化 |
| 收稿时间： | 2017-09-27 |
Isolation, Purification and Identification of Anti-tumor Bioactive Peptides from Chinemys reevesii |
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| SHI Yang,ZHANG Yong-jin,LAI Nian-yue,LIN Lin,JIANG Shao-tong and LU Jian-feng. Isolation, Purification and Identification of Anti-tumor Bioactive Peptides from Chinemys reevesii[J]. Modern Food Science & Technology, 2018, 34(5): 24-31 |
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| Authors: | SHI Yang ZHANG Yong-jin LAI Nian-yue LIN Lin JIANG Shao-tong LU Jian-feng |
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| Affiliation: | (1.School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China),(1.School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China),(2.Animal Husbandry and Fisheries Technology Promotion Center of Hefei, Hefei 231000, China),(1.School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China),(1.School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China) and (1.School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China) |
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| Abstract: | |
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| Keywords: | Chinemys reevesii anti-tumor anti-cancer bioactive peptides separation purification |
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