响应面优化金柑多酚的提取工艺

以金柑为实验材料,对金柑中多酚提取的最佳工艺条件进行研究。在单因素(提取时间、提取液浓度和液料比等)实验考察的基础上,以多酚含量为响应值,通过响应面法对金柑中游离态多酚以及结合态多酚的提取条件进行优化。实验结果表明,所得到的金柑游离态多酚及结合态多酚的工艺的回归模型显著,拟合性好,均可用于预测游离态多酚以及结合态多酚含量。优化后的游离态多酚提取条件如下:丙酮浓度77%,液料比33 mL/g,提取时间48 min,此条件下的游离态多酚平均含量为11.03 mg/g DW;优化后的结合态多酚提取条件为:NaOH浓度6.5 mol/L,液料比10 mL/g,提取时间18 h,此条件下的结合态多酚平均含量为1.79 mg/g DW。最优条件下的游离态多酚以及结合态多酚含量与模型预测值相符,表明优化的金柑多酚提取工艺合理。

Optimization of Extraction Process of Polyphenols from Kumquat by Response Surface Methodology

The optimum extraction process of polyphenols from Kumquat was investigated. Based on singe factor experiments (extract time, extract concentration, ratio of liquid to material, etc.), the extraction conditions of free polyphenols and bound polyphenols in kumquat were optimized by response surface methodology using polyphenol content as response value. The results showed that the established regression models of the free polyphenols and bound polyphenols of kumquat were significant and it could be used to predict the yield of free polyphenols and bound polyphenols. The optimum extraction conditions of free polyphenols were as follows: the acetone concentration of 77%, the liquid to material ratio of 33 mL/g; the extraction time of 48 min. The average free polyphenol content under this condition was 11.03 mg/g DW. The optimum extraction conditions of bound polyphenols were as follows: the NaOH concentration of 6.5 mol/L; the liquid to material ratio of 10 mL/g; the extraction time was 18 h. Under these optimized conditions, the yield of bound polyphenols reached 1.79 mg/g DW. The content of free polyphenols and bound polyphenols under optimal conditions were well matched with the model predicted yield, indicating that the optimized extraction process of free and bound polyphenols from kumquat was reasonable.