云南武定鸡、盐津乌骨鸡和大围山微型鸡肌肉中脂肪酸的组成分析

以云南省特有的三个优质地方鸡品种:武定鸡、盐津乌骨鸡、大围山微型鸡为研究对象,用溶剂提取法提取3种鸡胸肌及腿肌中总脂质,经甲脂化后,利用气相色谱-质谱(GC-MS)对脂肪酸组成及含量进行分析。结果显示,在云南武定鸡、盐津乌骨鸡和大围山微型鸡胸肌及腿肌中共含有20种脂肪酸,主要包括油酸、亚油酸、棕榈酸、硬脂酸、花生四烯酸。武定鸡与盐津乌骨鸡胸肌中总脂肪含量分别为28.470与25.042 mg/g,显著高于大围山微型鸡的16.769 mg/g(p0.05),大围山微型鸡腿肌中总脂肪含量为51.10 mg/g,显著高于武定鸡40.994 mg/g(p0.05);三种鸡的脂肪酸种类相同,饱和、不饱和脂肪酸占总脂肪酸比例相似,但各脂肪酸含量有所差异,三个鸡种腿肌中脂肪酸含量显著高于胸肌中脂肪酸含量(p0.05)。

Muscle Fatty Acids Analysis of Yunnan Wuding Chicken, Yanjin Silky Fowl and Daweishan Miniature Chicken

In this study, the total lipid was extracted by solvent extraction from breast and leg meat of Wuding chicken, Yanjin silky fowl and Daweishan miniature chicken.s. The fatty acids composition and contents were analyzed by use gas chromatography-mass spectrometry (GC-MS) after methyl esterification. Results showed that totally 20 kinds of fatty acids were identified in the breast and leg meat of Wuding chicken, Yanjin silky fowl and Daweishan miniature chickens, mainly including cis-9-oleic acid methyl ester, linoleic acid methyl ester, stearic acid methyl ester, palmitic acid methyl ester and arachidonic acid. The fatty acids contents of breast meats in Wuding chicken (28.470 mg/g) and Yanjin silky fowl (25.042 mg/g) were 28.470 and 25.042 mg/g, respectively, which were significant higher than that in Daweishan miniature chicken (16.769 mg/g). The fatty acids content of leg meat in Daweishan miniature chicken (51.10 mg/g) was significant higher than that in Wuding chicken (40.994 mg/g) (p<0.05).The same fatty acid composition in the three kinds of chickens was found with the similar saturated and unsaturated fatty acids proportion. However, the fatty acid contents in the three kinds of chickens had significant difference, and the fatty acids contents of leg meats were significant higher than those of breast meat in the three kinds chickens (p<0.05).