不同茶树品种鲜叶固定样的体外抗氧化活性

选用6种不同类型茶树品种一芽二叶鲜叶固定样,以Folin-Ciocalteu(福林酚)法测定其茶多酚含量,高效液相色谱(HPLC)法测定儿茶素、咖啡碱和没食子酸含量,三氯化铝法测定总黄酮含量,比较了不同品种茶叶鲜叶的主要抗氧化成分含量的差异。并通过分析其还原能力和清除DPPH自由基、亚硝基以及羟自由基(-OH)的实验研究,探讨了龙井群体种,龙井43号,云南群体种,云抗10号,安化群体种,槠叶齐6种不同茶树类型的体外抗氧化性能。实验结果表明,安化群体种的茶多酚(23.66±0.02%)和儿茶素(12.84±0.11 mg/mL)含量明显高于其他品种;龙井群体种,龙井43号,云南群体种,云抗10号,安化群体种,槠叶齐6个品种类型均具有较好的抗氧化活性,其中安化群体种的抗氧化活性更强。

Study on the Antioxidant Activity of Deffrent Tea Cultivars in Vitro

One bud and two leaves of six different types of tea fresh leaves were fixed. with for the determination of The content of tea polyphenol was determined by Folin-Ciocalteu (Folin phenol) method, and caffeine and catechin, gallic acid were determined by high performance liquid chromatography (HPLC) method. The contents of total flavonoids were determined by AlCl3 method to compare the differences of the main antioxidant components content in fresh leaves among different varieties of tea. Through the analysis of the reducing power and scavenging of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, nitroso and hydroxyl radical (?OH) experiment, we had discussed the in vitro antioxidant properties of six different types of tea (including Longjing Group, Longjing 43, Yunnan Group, Yunkang 10, Zhuyeqi, and Anhua Group). The results showed that the contents of tea polyphenols (23.66 ± 0.02%) and catechins (12.84 ± 0.11 mg/mL) in Anhua Group were significantly higher than those in other varieties. Longjing Group, Longjing 43, Yunnan Group, Yunkang 10, Anhua Group, and Zhuyeqi varieties all had good antioxidant activity, among which Anhua Group had stronger antioxidant activity than other varieties.