Modeling of Enzymatic Hydrolysis of Whey Proteins |
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Authors: | Gabriela Martínez-Araiza Eduardo Castaño-Tostado Silvia L Amaya-Llano Carlos Regalado-González Carlos Martínez-Vera Lech Ozimek |
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Affiliation: | 1.Programa de Posgrado en Alimentos del Centro de la República (PROPAC),Universidad Autónoma de Querétaro,Querétaro,Mexico;2.Departamento de Ingeniera de Procesos e Hidráulica,Universidad Autónoma Metropolitana–Iztapalapa,México,Mexico;3.Department of Agricultural, Food and Nutritional Science,University of Alberta,Edmonton,Canada |
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Abstract: | The aim of this work was to emphasize the limitations of modeling complex phenomena under unrealistic model assumptions. As a case study, the whey protein hydrolysis mechanism was modeled. A stirred batch reactor was used to study the degree of hydrolysis of sweet whey protein concentrate by using the protease alcalase. A completely random two-factorial experimental design was used, three levels of initial enzyme concentrations (E 0) (1.58, 3.18, 6.36 AU (Anson units)/L) times three levels of initial substrate concentrations (S 0) (18.73, 38.45, 81.16 g/L). All treatments were carried out at optimal alcalase—activity conditions: pH 8 and 50 °C. Reactions were monitored for 180 min. The degree of hydrolysis (h) curves was finally adjusted for each treatment to the exponential model using nonlinear regression techniques but not assuming a Michaelis–Menten relationship. From the estimation process, the coefficient b was constant (27.26 ± 1.37) and independent of E 0 and S 0, while coefficient a depended directly on the ratio E 0/S 0, ranging from 0.0017 to 0.0497. A noncritical strategy of forward modeling based on unrealistic assumptions was misleading in the face of complex phenomena; instead, a modeling strategy moving from data to the identification and estimation of parameters of practical interest must be considered. |
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