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Short communication: characterization of a kappa-casein genetic variant in the Chinese yak, Bos grunniens |
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| Authors: | Bai W L Yin R H Zhao S J Zheng Y C Zhong J C Zhao Z H |
| Affiliation: | * Animal Science and Veterinary Medicine College, Shenyang Agricultural University, Shenyang 110161, China † Animal Science and Veterinary Medicine College, Jilin University, Changchun 130062, China ‡ Animal Science Research Academy of Sichuan Province, Chengdu 610066, China § Life Science and Technology College, Southwest University for Nationalities, Chengdu 610041, China |
| Abstract: | A PCR-single strand conformation polymorphism protocol has been developed for rapid genotyping of the yak κ-casein gene. A total of 307 yaks from the Tianzhu White, Jiulong, Maiwa, and Datong breeds in China were genotyped at the κ-casein locus using the protocol developed in the present study. A polymorphism of κ-casein gene exon 4 has been identified in Tianzhu White breed by evaluating genomic DNA. The polymorphic site consists of a single nucleotide substitution G→C at position 362 of the exon 4, resulting in an AA substitution from Arg to Pro at position 121 of the AA sequence and in 2 alleles named, respectively, G and C based on nucleotide 362. The occurrence of allele C in the Tianzhu White breed was high with an allele frequency of 0.15. However, allele C appears to be absent in the yaks from Jiulong, Maiwa, and Datong breeds. |
| Keywords: | genetic polymorphism yak κ-casein |
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