双卤代均三嗪活性染料及其水解产物的 HPLC 分离方法的建立

为了研究双卤代均三嗪活性染料的水解反应，首先采用反相离子对高效液相色谱对双卤代均三嗪活性染料及其水解产物进行了色谱分离。从双卤代均三嗪活性染料的选择、色谱柱的选择、流动相的选择和优化、柱温的确定等方面，探讨了对称结构双卤代均三嗪活性染料及其水解产物的HPLC分离方法的建立过程，利用该方法对双一氟均三嗪活性染料活性红LS-2G及双一氯均三嗪活性染料活性红KE-3B的水解产物进行了分离，得到了较好的分离效果。

Establishment of HPLC separation method for bis (halogeno-s-triazine) reactive dyes and their hydrolysates

In order to study the hydrolysis reaction of bis(halogeno-s-trazine) reactive dyes, reversed-phase ion-pair high performance liquid chromatographic was employed to separate the bis(halogeno-s-trazine) reactive dyes and their hydrolysis products. From some aspects such as the selection of the bis(halogeno-s-trazine) reactive dyes and chromatographic column, selection and optimization of mobile phase, and determination of column temperature, and so on, a establishing procedure of HPLC separation method that appropriated for bis(halogeno-s-trazine) reactive dyes and their hydrolysis products was discussed. By this method the bis(monofluo-s-trazine) reactive dye Reactive Red LS-2G and bis(monochloro-s-trazine) reactive dye Reactive Red KE-3B and their hydrolysis products were separated completely.