|
|||||
|
|
| 烟草青枯菌拮抗放线菌的筛选及鉴定 | |
| 引用本文： | 陆铮铮,彭丽娟,丁海霞,左希,彭杰,蒋选利. 烟草青枯菌拮抗放线菌的筛选及鉴定[J]. 中国烟草科学, 2013, 34(2): 54-58. DOI: 10.3969/j.issn.1007-5119.2013.02.012 |
| 作者姓名： | 陆铮铮 彭丽娟 丁海霞 左希 彭杰 蒋选利 |
| 作者单位： | 1. 贵州大学贵州山地农业病虫害重点实验室,贵阳 550025; 2. 贵州大学农学院,贵阳 550025; 3. 中国农业大学农学与生物技术学院,北京 100193; 4. 咸阳市城市绿化管理处,陕西 咸阳 712000; 5. 天柱县烟草专卖局,贵州 天柱 556600 |
| 基金项目： | 中国烟草总公司贵州省公司科技项目(200917);铜仁地区烟草公司项目[贵铜烟(2009)10] |
| 摘 要： | 为筛选出烟草青枯菌的有效拮抗菌,本研究从烟草根围土壤中分离了97株放线菌,通过平板对峙培养法筛选出抑菌圈直径均达20 mm以上的拮抗放线菌3株.根据其在鉴别培养基上的培养特征、孢子和孢子链的形态特征,生理生化特性以及16SrDNA序列分析对这3株拮抗放线菌进行鉴定.结果表明,3株拮抗放线菌都属于链霉菌属(Streptomyces),分别为粉红孢类群中的玫瑰暗黄链霉菌(S. roseofulvus Preobrazhenskaya)、绿色类群中的橄榄绿链霉菌(S. olivaceoviridis Preobrazhenskaya)和灰褐类群中的黄麻链霉菌(S. corchorusii Ahmed).其中,玫瑰暗黄链霉菌菌株对青枯劳尔氏菌的拮抗效果最好,其平均抑菌圈直径可达54.66 mm;橄榄绿链霉菌菌株的拮抗效果次之,其平均抑菌圈直径为43.20 mm;黄麻链霉菌平均抑菌圈直径为20.34 mm. |
| 关 键 词： | 烟草 青枯病 生物防治 放线菌 |
| 收稿时间： | 2012-02-20 |
Screening and Identifying of Antagonistic Actinomycetes against Ralstonia Solancearum |
|
| LU Zhengzheng,PENG Lijuan,DING Haixia,ZUO Xi,PENG Jie,JIANG Xuanli. Screening and Identifying of Antagonistic Actinomycetes against Ralstonia Solancearum[J]. Chinese Tobacco Science, 2013, 34(2): 54-58. DOI: 10.3969/j.issn.1007-5119.2013.02.012 | |
| Authors: | LU Zhengzheng PENG Lijuan DING Haixia ZUO Xi PENG Jie JIANG Xuanli |
| Affiliation: | 1. Guizhou Key Laboratory for Plant Pest Management of Mountainous Region, Guizhou University, Guiyang 550025, China;2. Agricultural College of Guizhou University, Guiyang 550025, China;3. College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China;4. City Greening Management of Xianyang, Xianyang, Shanxi 712000, China;5. Tianzhu County Tobacco Company of Guizhou Province, Tianzhu, Guizhou 556600, China |
| Abstract: | In order to select effective antagonistic actinomycetes against Ralstonia solanacearum, we isolated 56 strains of actinomycetes from healthy tobacco rhizosphere soils. By pairing culture on plate method to screen antagonistic actinomycetes, we attained 3 strains of antagonistic actinomycetes, whose average inhibition diameters were more than 20 mm. We identified 3 strains of actinomycetes according to culture characteristics on differential culture mediums, spore and spore chain morphology, physiological and biochemical, molecular biology methods. The results showed that the 3 strains of actinomycetes were Streptomyces roseofulvus, Streptomyces olivaceoviridis and Streptomyces corchorusii. Among them, the best was S. roseofulvus, and average inhibition diameter was 54.66 mm. Average inhibition diameter of S.olivaceoviridis was 43.20 mm, and average inhibition diameter of S. corchorusii was 20.34 mm. |
| Keywords: | tobacco bacterial wilt biocontrol actinomycete |
| 点击此处可从《中国烟草科学》浏览原始摘要信息 | |
| 点击此处可从《中国烟草科学》下载全文 | |