硫杆菌的耐高砷诱变及对高砷铜精矿的浸出

为提高硫杆菌浸出铜过程中的耐砷能力，对用高砷铜精矿驯化的中温硫杆菌进行了紫外线耐高砷诱变，选育出的中温硫杆菌耐砷能力达8．0g／L，而未经诱变的对照菌只能在As^3＋含量小于1．0g／L的环境中生长繁殖。用选育出的耐砷诱变菌对含As3％-8％的高砷铜精矿进行铜的细菌浸出试验，结果表明。精矿粒度为-250目、矿浆浓度为10％-15％、砷含量不超过5％的铜精矿细菌浸出效果较好，铜浸出率可达77％以上；添加Ag^＋和对浮选铜精矿进行脱药处理均能不同程度地强化细菌浸出效果。

Arsenic-tolerant Mutation of Thiobacillus Ferrooxidans and Its Application in Leaching of High Arsenic-Containing Copper Concentrate

To raise the arsenic-tolerant ability of thiobacillus ferrooxidans in the copper leaching process,ultraviolet ray arsenic-tolerant mutation was made on the medium temperature thiobacillus ferrooxidans acclimated by high arsenic copper concentrate.The isolated medium temperature thiobacillus ferrooxidans had an arsenic-tolerant ability of 8.0 g/L while the unmutated thiobacillus ferrooxidans could only grow and reproduce in an environment with As~(3 ) content of only below(1.0) g/L.The arsenic-tolerant mutant was used in the copper bio-leaching of high arsenic-containing copper concentrate with an As content of 3% ~ 8%.The results showed that the better bio-leaching effect could be achieved at a concentrate particle size of-250 mesh,pulp density of 10% ~15% and an arsenic content of not more than 5%.The copper leaching rate could be over 77% and the addition of Ag~ and the removal of the flotation reagent could intensify the bio-leaching effect in various degrees.