The detection of aflatoxin B1 in peanut kernels,peanut butter and maize using a monoclonal antibody based enzyme immunoassay |
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| Affiliation: | 1. Department of Evolutionary Biology, Ecology and Environmental Sciences, University of Barcelona, Institute of Biomedicine of the University of Barcelona (IBUB), Av Diagonal 643, 08028 Barcelona, Spain;2. Mental Health Networking Biomedical Research Centre (CIBERSAM), Spain;3. Department of Psychology, University of Lleida. Campus de Cappont, Av de l''Estudi General 4, 25001 Lleida, Spain;4. Department of Basic and Clinical Psychology and Psychobiology, Universitat Jaume I, Av de Vicent Sos Baynat s/n, 12071 Castelló de la Plana, Spain;5. FIDMAG Germanes Hospitalàries Research Foundation, C/ Doctor Antoni Pujadas 38, 08830 Sant Boi de Llobregat (Barcelona), Spain |
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| Abstract: | A simple procedure was validated for the routine immunochemical analysis of AFB1 in peanut kernels, peanut butter and maize. The specificity, affinity and sensitivity of the monoclonal antibody (McAb) employed was such that minimal sample preparation was required. The enzyme immunoassay (EIA) had a sensitivity for standard AFB1 of 0·2 ng ml−1 with a working range up to 30 ng ml−1 and was not significantly affected by matrix interference of samples. Essential protocol features were (1) blending substrate with methanol: water; (2) filtering blended sample; and (3) analysis of filtrate by EIA after dilution with buffer. Average recoveries of AFB1 spiked samples at levels of 6–400 ppb were 90–112·5%. Using laboratory prepared samples contaminated with Aspergillus flavus there was high positive correlation (r = 0·97) when EIA results were compared with thin layer chromatography (TLC) techniques. |
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