| 一种新型溴氰菊酯农药半抗原的合成及应用效果 |
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| 引用本文： | 孔晔,李培武,张奇,张文,丁晓霞,黄艳玲,唐晓倩.一种新型溴氰菊酯农药半抗原的合成及应用效果[J].化学试剂,2009,31(4). |
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| 作者姓名： | 孔晔 李培武 张奇 张文 丁晓霞 黄艳玲 唐晓倩 |
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| 作者单位： | 1. 中国农业科学院油料作物研究所,湖北,武汉,430062
2. 中国农业科学院油料作物研究所,湖北,武汉,430062;农业部油料及制品质量监督检验测试中心,湖北,武汉,430062 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，国家自然科学基金，武汉市科技局项目 |
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| 摘 要： | 利用化学方法合成了一种溴氰菊酯新型半抗原,并通过核磁共振鉴定;用活泼酯法和EDC法将溴氰菊酯半抗原分别与牛血清白蛋白(BSA)和卵清白蛋白(OVA)偶联制备免疫抗原和包被抗原,紫外吸收法鉴定表明吸收峰出现在305nm,发生了红移,计算偶联比分别为7:1和6:1;免疫小鼠后获得抗体的效价为64 000,IC<,50>为0.16μg/mL.研究为研制溴氰菊酯农药残留免疫速测试剂,建立免疫速测技术奠定了基础.
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| 关 键 词： | 溴氰菊酯 半抗原 人工抗原 合成 抗血清 |
Synthesis and application of a novel hapten of pesticide deltamethrin |
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| KONG Ye,LI Pei-wu,ZHANG Qi,ZHANG Wen,DING Xiao-xia,HUANG Yan-ling,TANG Xiao-qian.Synthesis and application of a novel hapten of pesticide deltamethrin[J].Chemical Reagents,2009,31(4). |
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| Authors: | KONG Ye LI Pei-wu ZHANG Qi ZHANG Wen DING Xiao-xia HUANG Yan-ling TANG Xiao-qian |
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| Affiliation: | 1.Oil Crops Research Institute;CAAS;Wuhan 430062;China;2.Quality Inspection and Test Center for Oilseeds & Products;Agricultural Ministry;China |
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| Abstract: | A novel hapten of deltamethrin was synthesized and its structure was verified by 1HNMR spectrometry.By using active ester and EDC method,the hapten was conjugated with BSA and OVA to prepare the immune antigen and coating antigen,respectively.Results of the UV absorption spectroscopy showed that the absorption peak was found to have a bathochromic shift at 305 nm,and the calculated coupling ratio was 7:1 and 6:1,respectively.After immunizing mouse with the hapten,a high selective antibody against deltamethr... |
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| Keywords: | deltamethrin hapten artificial antigen synthesis antibody |
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