Preparation and characterization of magnetic nanoparticles embedded in hydrogels for protein purification and metal extraction |
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Authors: | Narayana Reddy Nagireddy Murali Mohan Yallapu Varaprasad Kokkarachedu Ravindra Sakey Vimala Kanikireddy Joy Pattayil Alias Mohana Raju Konduru |
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Affiliation: | (1) Synthetic Polymer Laboratory, Department of Polymer Science and Technology, Sri Krishnadevaraya University, Anantapur, 515 055, AP, India;(2) Physical & Materials Chemistry Division, National Chemical Laboratory, Pune, India;(3) Present address: Cancer Biology Research Center, Sanford Research/USD, Sioux Falls, SD 57105, USA; |
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Abstract: | The present work involves the development of hydrogel magnetic nanocomposites for protein purification and heavy metal extraction
applications. The magnetic nanoparticles (MNPs) were prepared in situ in poly(acrylamide)-gum acacia (PAM-GA) hydrogels. The
formation of magnetic nanoparticles in the hydrogel networks was confirmed by Fourier transform infrared (FTIR) spectroscopy
and X-ray diffraction (XRD). Scanning electron (SEM) microscopy studies revealed the formation of MNPs throughout the hydrogel
networks. The average size of MNPs formed in the hydrogel networks was 3–5 nm as determined by transmission electron microscopy
(TEM). The thermal properties of the hydrogel magnetic nanocomposites were evaluated by dynamic scanning calorimetry (DSC)
and thermogravimetric (TG) analysis. The magnetic properties of the developed hydrogel magnetic nanocomposites were determined
by a vibrating sample magnetometer (VSM). The swelling properties of the hydrogel and the hydrogel magnetic nanocomposites
were studied in detail. The hydrogel magnetic nanocomposites are utilized for the removal of toxic metal ions such as Co(II),
Ni(II), and Cu(II) and for protein purification. The results confirm that the hydrogel magnetic nanocomposites exhibit superior
extraction properties to hydrogels. |
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