| 食品中大肠杆菌O157:H7的几种检测方法的比较 |
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| 引用本文： | 周勇,张晶,侯水平,邓志爱,吴新伟,陈守义,杨智聪. 食品中大肠杆菌O157:H7的几种检测方法的比较[J]. 中国食品卫生杂志, 2012, 0(1): 27-30 |
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| 作者姓名： | 周勇 张晶 侯水平 邓志爱 吴新伟 陈守义 杨智聪 |
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| 作者单位： | 广州市疾病预防控制中心 |
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| 基金项目： | 广州市医药卫生科技项目(2009-YB-125);广州市卫生局医药卫生科技重点项目(2006-Zdi-11) |
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| 摘 要： | 目的评估平板分离培养法、免疫磁珠分离(IMS)法、VIDAS全自动酶标免疫测试系统、BAX全自动病原菌检测系统及环介导等温扩增(LAMP)技术在食品中检验肠出血型大肠埃希菌O157∶H7的特异性、敏感性。方法使用平板分离培养法、免疫磁珠分离法、VIDAS法、BAX法及LAMP法对人工制备的染菌猪肉样本进行检测,并对这几种方法进行比较。结果 BAX法和LAMP法的检出率最高,分别是89.1%和85.9%,免疫磁珠法和VIDAS法检出率次之,分别是75.0%和78.1%,传统分离培养法为43.8%。结论 BAX法和LAMP法具有快速、高效、特异性好、敏感性高的特点,可快速筛选食品中可能存在的肠出血型大肠埃希菌O157∶H7。
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| 关 键 词： | 大肠杆菌O157:H7 BAX全自动病原菌检测系统 环介导等温扩增 检测方法 食源性致病菌 |
Comparison of methods detecting Escherichia coli O157:H7 in foods |
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| Zhou Yong,Zhang Jing,Hou Shuiping,Deng Zhiai,Wu Xinwei,Chen Shouyi,Yang Zhicong. Comparison of methods detecting Escherichia coli O157:H7 in foods[J]. Chinese Journal of Food Hygiene, 2012, 0(1): 27-30 |
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| Authors: | Zhou Yong Zhang Jing Hou Shuiping Deng Zhiai Wu Xinwei Chen Shouyi Yang Zhicong |
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| Affiliation: | (Guangzhou Center for Disease Control and Prevention,Guangzhou 510440,China) |
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| Abstract: | Objective To examine the specificity and sensitivity of traditional cultural method,immunomagnetic beads separation method(IMS),VIDAS,BAX and Loop-mediated isothermal amplification(LAMP) method for the detection of O157∶H7 in foods.Methods Traditional cultural method,IMS,VIDAS,BAX and LAMP method were used and compared in detecting O157∶H7 in pork samples.Results The detection rate of BAX,LAMP,IMS and VIDAS method was 89.1%,85.9%,75.0% and 78.1% respectively,while that of traditional cultural method was only 43.8%.Conclusion BAX and LAMP method were proved to be rapid,highly effective,specific and sensitive for the detection of O157∶H7 in foods. |
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| Keywords: | Escherachia coli O157∶H7 BAX loop-mediated isothermal amplification(LAMP) detection methods foodborne pathogens |
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