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Rapid Determination of Spinal Cord Content in Ground Beef by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy
Authors:RR Gangidi    A Proctor    FW Pohlman
Affiliation:Authors Gangidi and Proctor are with the Dept. of Food Science, 2650 N. Young Ave., Univ. of Arkansas, Fayetteville, AR 72704. Author Pohlman is with the Dept. of Animal Science, Univ. of Arkansas, Fayetteville. Direct inquiries to author Proctor (E-mail: ).
Abstract:ABSTRACT: The potential of attenuated total reflectance Fourier transform infrared spectroscopy to measure spinal cord in ground beef was investigated. Mid‐infrared spectra were obtained for ground beef adulterated with 0 to 100 ppm spinal cord. Partial least squares models were developed using 400 to 4000 cm‐1. The model in the 400 to 4000 cm‐1 range showed a calibration of Rcall= 0.94 (RMSE = 11.24) and validation of Rval= 0.87 (RMSE = 17.22). The P‐O‐C and N‐H stretches were found to correlate positively with spinal cord content. Fourier transform infrared spectroscopy has the potential to rapidly determine spinal cord adulteration in ground beef.
Keywords:spinal cord  ground beef  sphingomyelin  ATR-FTIR  PLS
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