Analysis of heat-treated bovine cortical bone by thermal gravimetric and nanoindentation |
| |
Affiliation: | 1. Department of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, NY, USA;2. Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, USA;1. Key Laboratory of Advanced Textile Materials and Manufacturing Technology of Ministry of Education, College of Materials and Textile, Zhejiang Sci-Tech University, Hangzhou 310018, China;2. Institute of Biomaterials and Marine Biological Resources, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China;3. Institute of Natural Science, Yonsei University, Seoul 120-749, Republic of Korea;1. Department of Biomedical Engineering, Vanderbilt University, Nashville, TN 37232, United States;2. Department of Orthopaedic Surgery & Rehabilitation, Vanderbilt University Medical Center, Nashville, TN 37232, United States;3. Center for Bone Biology, Vanderbilt University Medical Center, Nashville, TN 37232, United States;4. University of Kentucky Barnstable Brown Diabetes Center, Lexington, KY 40536, United States;5. Department of Pediatrics, University of Kentucky College of Medicine, Lexington, KY 40536, United States;6. Department of Medicine, Division of Nephrology, Vanderbilt University Medical Center, Nashville, TN 37232, United States;7. Department of Veterans Affairs, Tennessee Valley Healthcare System, Nashville, TN 37212, United States;8. Center for Matrix Biology, Vanderbilt University Medical Center, Nashville, TN 37232, United States |
| |
Abstract: | Xenograft bone has been widely used as a bone grafting material because it gains advantages in biological and mechanical properties as compare with the use of an allograft bone. Heat-treatment of bone is recognized as one of the simple and practical methods to lower the human immunodeficiency virus (HIV) infection and overcome the risks of rejection and disease transfer during the bone transplantation. Therefore, understanding the change of bone’s organic matrix after heat treatment has become a significant topic. In this study, thermal gravimetric analysis (TGA) was used to investigate the condition of organic constituents of a bovine cortical bone. In order to well characterize the microstructural and mechanical property of the bone after heat treatment, nanoindention technique was also employed to measure the localized elastic modulus (E) and hardness (H) of its interstitial lamellae and osteons lamellae at the temperatures of 23 °C (RT), 37 °C, 90 °C, 120 °C and 160 °C, respectively.The TGA results demonstrated that heat-treated bones had three stages of weight loss. The first stage was the loss of water, which started from RT to 160 °C. Follow by a weight loss of organic constituents starting from 200 °C to 600 °C. Upon reaching 600 °C, the organic constituents were decomposed and mineral phase loss started taking place until 850 °C. From the nanoindentation results, it showed the values of E and H measured for the interstitial lamellae were higher than that of the osteons lamellae. This phenomenon indicates that the interstitial lamellae are stiffer and easy to be mineralized than osteons lamellae. For a specimen heat-treated at 90 °C, the values of E and H of interstitial lamellae and osteons lamellae were similar to a non-heat-treated specimen. For a specimen heat-treated at 120 °C, its interstitial lamellae had higher E and H values than osteons lamellae. When a specimen was heat-treated at 160 °C, both interstitial lamellae and osteons lamellae demonstrated a slight decrease of their E and H values. An ANOVA statistical analysis was used to analyze the difference in elastic properties and hardness in various temperature ranges. |
| |
Keywords: | Bovine cortical bone A. Nano-structures B. Mechanical properties D. Non-destructive testing D. Thermal analysis |
本文献已被 ScienceDirect 等数据库收录! |
|