| 单扫描极谱法快速测定蛋白质的研究 |
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| 引用本文： | 李耀仓,马红霞.单扫描极谱法快速测定蛋白质的研究[J].云南化工,2006,33(4):43-46. |
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| 作者姓名： | 李耀仓 马红霞 |
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| 作者单位： | 1. 武汉生物工程学院,应用化学系,湖北,武汉,430415
2. 湖北省化学研究院,光通讯材料中心,湖北,武汉,430074 |
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| 摘 要： | 在pH 4.7的HAc-NaAc缓冲溶液中,邻二氮菲与蛋白质相互作用,使邻二氮菲在-0.99 V(vs.SCE)处的还原峰电流下降,电流降低值与所加的蛋白质(人血清白蛋白、牛血清白蛋白、溶菌酶)的量在一定范围内呈线性关系,线性范围分别为2.0~22 mg/L,2.0~20 mg/L和4.0~26 mg/L;检测限分别为1.0 mg/L,1.0 mg/L,2.0mg/L。运用该方法测定了人血清中白蛋白的含量,结果满意。
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| 关 键 词： | 单扫描极谱法 牛血清白蛋白(BSA) 人血清白蛋白(HSA) 邻二氮菲(Phen) |
| 文章编号： | 1004-275X（2006）04-0043-04 |
| 收稿时间： | 2006-05-30 |
| 修稿时间： | 2006年5月30日 |
Fast Determination of Proteins by Single Sweep Polarography |
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| LI Yao-cang,MA Hong-xia.Fast Determination of Proteins by Single Sweep Polarography[J].Yunnan Chemical Technology,2006,33(4):43-46. |
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| Authors: | LI Yao-cang MA Hong-xia |
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| Affiliation: | 1. Department of Applied Chemistry, Wuhan Bioengineering Institute, Wuhan 430415, China; 2. Center of Optical Communication Material, Huboi Research Institute of Chemistry, Wuhan 430074, China |
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| Abstract: | Interaction between proteins(BSA,HSA or Lyso) and phenanthroline hydrate was studied in HAc-NaAc buffer solution at pH 4.7 with single sweep polarography.The reduction peak current of phenanthroline at-0.99 V(vs.SCE) decreased and the decreased value is linear to the adding proteins.The linear ranges 2.0~22μg/ml for HSA,2.0 ～20μg/ml for HAS and 4.0～26μg/ml for Lyso and the detection limit were 1.0,1.0 and 2.0μg/ml for BSA,HSA and Lyso respectively.The method was used for the determination of total protein from human serum and the results were satisfactory. |
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| Keywords: | single sweep polarography bovine serum albumin(BSA) human serum albumin(HSA) phenanthroline hydrate(phen) |
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