| 雌二醇诱导人正常肝细胞的凋亡以及对细胞周期的影响 |
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| 引用本文： | 李宁,于力方,王珊,廖杰. 雌二醇诱导人正常肝细胞的凋亡以及对细胞周期的影响[J]. 现代科学仪器, 2011, 0(5): 121-123 |
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| 作者姓名： | 李宁 于力方 王珊 廖杰 |
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| 作者单位： | 解放军总医院医学实验测试中心,北京,100853 |
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| 摘 要： | 目的：观察雌二醇对诱导人正常肝细胞的的凋亡,并且研究其对细胞周期的影响。方法：采用人肝细胞为实验对象,用he染色观察细胞形态学变化;流式细胞记数仪检测细胞周期,AnnexinⅤ/PI双重染色定量检测早期细胞凋亡及Bcl-2/Bax的表达改变。结果：雌二醇48h可以明显抑制正常肝细胞株的增生与活力,诱导细胞的凋亡,且对细胞周期的影响非常显著。结论：其机制可能与Bcl-2/Bax表达的下调有关。
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| 关 键 词： | 雌二醇 cas雌二醇se-9 流式细胞检测 细胞周期 凋亡 肝细胞 |
Apoptosis of normal Liver cells Induced by Estradiol and Influence for Cell Cycle |
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| Li Ning,Yu Lifang,Wang Shan,Liao Jie. Apoptosis of normal Liver cells Induced by Estradiol and Influence for Cell Cycle[J]. Modern Scientific Instruments, 2011, 0(5): 121-123 |
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| Authors: | Li Ning Yu Lifang Wang Shan Liao Jie |
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| Affiliation: | Li Ning,Yu Lifang,Wang Shan,Liao Jie(Medical Experiment and Analysis Center,PLA General Hospital,Beijing 100853 China) |
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| Abstract: | Objective: apoptosis of normal liver cells induced by estradiol is observed,the influence for the cell cycle is analyzed;Methods: Person liver cell was used for experimental object,He was used to dyed for observation morphological changes of cells;Flow cytometric numeration detection instrument was used to determine cell cycle,V/PI double dyeing was used to detecte early cell apoptosis,and the change of Bcl-2 / Bax expression;Results: Estradiol 48h can significantly inhibit the normal liver cell proliferati... |
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| Keywords: | Estradiol Cas estradiol se-9 Streaming cell detection The cell cycle Apoptosis Liver cell |
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