Establishment of the enzyme-linked immunosorbent assay for connective tissue growth factor (CTGF) and its detection in the sera of biliary atresia |
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Authors: | T Tamatani H Kobayashi K Tezuka S Sakamoto K Suzuki T Nakanishi M Takigawa T Miyano |
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Affiliation: | Pharmaceutical Frontier Research Laboratories, JT Inc., Yokohama, Kanagawa, 236-0004, Japan. tamatani@ikrl.jti.co.jp |
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Abstract: | Connective tissue growth factor (CTGF) is a mitogenic, chemotactic, and cell matrix-inducing factor for fibroblasts. We generated murine monoclonal antibodies against CTGF and established a sandwich enzyme-linked immunosorbent assay (ELISA) for detection of CTGF. By using the ELISA, we confirmed that CTGF was specifically induced in human fibroblasts by TGF-beta but not by PDGF, FGF, IGF-I, or EGF. We also found that the serum levels of CTGF were significantly correlated with the progression of hepatic fibrosis in biliary atresia. These results indicated that CTGF is potentially a useful parameter for monitoring certain types of fibrotic disorders. |
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