Quick method for estimating free gossypol in cottonseed,meats, collets,and extracted meals |
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Authors: | R J Hron Sr M S Kuk P J Wan |
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Affiliation: | (1) SRRC, ARS, USDA, 70179 New Orleans, Louisiana;(2) Southern Regional Research Center, P. O. Box 19687, 70179 New Orleans, LA |
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Abstract: | A method for estimating free gossypol (FG) has been developed that decreases sample-determination time from over 2 h to about
25 min per sample. With auto pipetters and bottle-to dispensers, six sample determinations can be completed in approximately
50 min. The method consisted of adding water and acetone separately to a fixed sample weight, mixing, filtering, diluting
with 65% acetone, and reading absorbance on a spectrophotometer. Absorbance was plotted against the official American Oil
Chemists’ Society’s FG method for samples that contained FG between 0.02 and 0.9%. Quadratic least squares regression for
31 samples had a correlation coefficient ofr
2=0.986 and a standard error of estimated FG of 0.032%.
Presented in part at the AOCS Annual Meeting, Anaheim, California, April 1993. |
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Keywords: | Analysis cottonseed environment gossypol plant breeder polyphenol spectrophotometer |
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