三苯基锡的微生物降解特性及降解菌差异表达蛋白

考察了克雷伯氏菌(Klebsiella pneumoniae)对浓度为0.1～10 mg·L^-1的三苯基锡(TPhT)的生物降解,分析了在无机盐培养基和去离子水中K.pneumoniae与TPhT作用过程中,其生理特性和蛋白组的变化,以期从降解菌蛋白质组以及菌体离子代谢的角度揭示有机锡的微生物降解机理。实验结果显示K.pneumoniae能有效地将TPhT降解为二苯基锡、一苯基锡和锡。TPhT在无机盐培养基和去离子水中的降解率分别为46.7%～73.6%和25.1%～87.0%。在此过程中TPhT增加了菌体细胞膜的通透性,从而引发细胞膜的损害,刺激了细胞内离子的释放,造成部分细胞的死亡。TPhT诱导菌体表达了部分与蛋白质水解、代谢过程、蛋白质运输、能量转换、TPhT抗性和降解有关的差异蛋白。

Triphenyltin biodegradation and differential expression proteins of degrading bacteria

To investigate the mechanism of triphenyltin(TPhT)biodegradation through proteomic and ions release,the biodegradation of TPhT with concentration from 0.1 to 10 mg·L^-1 by Klebsiella pneumoniae,and the physiological responses and proteomic changes of K.pneumoniae to TPhT exposure in mineral salts medium(MSM)and deionized distilled water(DDW)were studied.Experimental results showed that K.pneumonia could effectively degrade TPhT to diphenyltin(DPhT),monophenyltin(MPhT)and tin(Sn),and increase TPhT removal rate from 46.7% to 73.6% and from 25.1% to 87.0% in MSM and DDW,respectively.In this process,TPhT activated membrane permeability and triggered cell membrane damage,which increased the release of intracellular ions and caused part of cell death. Moreover,the exposure of K.pneumonia to TPhT induced differential expression of some proteins that were responsible for proteolysis,metabolic processes,protein translocation,energy conversion,TPhT stress tolerance,and TPhT elimination.