| 高效毛细管电泳-紫外检测法分离测定微波复合酶法大豆蛋白水解物中的多肽 |
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| 引用本文： | 陈均志,张海平,顾玲,陈立红.高效毛细管电泳-紫外检测法分离测定微波复合酶法大豆蛋白水解物中的多肽[J].食品工业科技,2005,26(8):163-165. |
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| 作者姓名： | 陈均志 张海平 顾玲 陈立红 |
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| 作者单位： | 陕西科技大学轻化工助剂省重点实验室,陕西,成阳,712081 |
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| 基金项目： | 陕西省教育厅重点资助项目(05JK107) |
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| 摘 要： | 以高效毛细管电泳(HPCE)无胶筛分方式(MEKC)对微波复合酶法大豆蛋白水解物中的多肽成功地进行了分离和检测,以100mmol/L Tris/HCl(pH8.5) 0.1%SDS 0.1%PEO为缓冲液,运行电压10kv。文章对分离的机理作了探讨,并对实验中出现的现象作了讨论。
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| 关 键 词： | 高效毛细管电泳 无胶筛分 大豆蛋白 紫外检测 |
| 文章编号： | 1002-0306(2005)08-0163-03 |
| 修稿时间： | 2005年1月4日 |
Separation and determination of peptides in soybean protein hydrolysate by microwave assisted enzymatic hydrolysis by high-performance capillary electrophoresis |
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| Chen Junzhi et al.Separation and determination of peptides in soybean protein hydrolysate by microwave assisted enzymatic hydrolysis by high-performance capillary electrophoresis[J].Science and Technology of Food Industry,2005,26(8):163-165. |
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| Authors: | Chen Junzhi |
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| Affiliation: | Chen Junzhi et al |
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| Abstract: | The article studies the isolation and identification of the protein polypeptides in soybean protein hydrolysate with microwave complex enzymes by non -gel sieving high -performance capillary electrophoresis. The running buffer was composed of 100mmol/L Tris/HCI(pH8.5) 0.1%SDS 0.1%PEO and the running voltage was 10kv. The separation mechanism and some phenomena during the experiment are discussed. |
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| Keywords: | high-performance capillary electrophoresis non-gel sieving high -performance capillary electrophoresis soybean protein ultraviolet detection |
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