检测器串联法测定婴幼儿配方乳粉中多种B族维生素

建立了准确、灵敏的婴幼儿配方乳粉中维生素B2、B3和B6的液相色谱同时测定方法。前处理中运用等电点法去除蛋白质干扰,在流动相中添加辛烷磺酸钠离子对试剂并对其pH值进行优化,通过梯度洗脱程序,实现了多组分的分离。采用二极管阵列检测器(DAD)和荧光检测器(FLD)串联双检测器定性和多通道定量。结果表明,目标物检出限在0.05～1.0 mg/kg,在各自浓度范围内线性良好,线性相关系数均大于0.999,加标回收率在92.5%～106.4%范围内,相对标准偏差为0.84%～1.45%。用于实际样品的检测,结果满意。

Simultaneous determination of B Vitamins in infant formula by UPLC-DAD/FLD

To establish a method for the simultaneous determination of B2,B3 and B6 vitamins in infant formula by ultra high performance liquid chromatography-diode array detector-fluorescence detector(UPLC-DAD/FLD).Methods:During the sample preparation,the pro-tein was removed by isoelectric point precipitation.The separation was proved to be practical by pH and organic solvent gradient elution for multicomponent simultaneous determination.The DAD/FLD were used in series to achieve qualitative and quantitative detection.Results:High correlation coefficients(R^2>0.999)for the vitamins in their linear ranges were obtained.The spiked recovery rates and accuracy(ex-pressed as relative standard deviation,RSD)were determined to be in the ranges of 92.5%~106.4%and 0.84%~1.45%.This method also has good precision and accuracy and can be applied to detect the vitamins in infant formula.