| 酶水解-液相色谱法测定婴幼儿食品中的色氨酸 |
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| 引用本文： | 孙立臻,别梅,冯峰,王骏,祝建华,刘艳明,张峰.酶水解-液相色谱法测定婴幼儿食品中的色氨酸[J].中国乳品工业,2019(9):47-51. |
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| 作者姓名： | 孙立臻 别梅 冯峰 王骏 祝建华 刘艳明 张峰 |
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| 作者单位： | 山东省食品药品检验研究院;山东省特殊医学用途配方食品质量控制工程技术研究中心;中国检验检疫科学研究院 |
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| 基金项目： | 食品安全关键技术研发重点专项(2017YFC1601600) |
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| 摘 要： | 利用酶解水解法,建立了婴幼儿配方乳粉(以下简称乳粉)和特殊医学用途婴儿配方食品(以下简称为医学食品)中游离和总色氨酸的高效液相色谱检测方法。样品经蛋白酶水解后,经C18色谱柱(150mm×4.6mm,3.5μm)分离,以甲醇-0.1%乙酸溶液作为流动相等度洗脱,紫外检测器串联荧光检测器检测,外标法定量。结果表明:在质量浓度为0.2~100μg/mL范围内线性关系良好(r2=0.9999)。添加质量分数在100,500,1000mg/100g时,色氨酸的回收率介于99.0%~105.3%之间。色氨酸的检出限为1.5mg/100g,定量限为5mg/100g。本方法操作简便,重现性好,可应用于市售乳粉和医学食品中色氨酸含量的测定,填补了检测标准缺失的空白。
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| 关 键 词： | 高效液相色谱法 色氨酸 乳粉 医学食品 酶水解 |
Detection of tryptophan in infant food by enzymatic hydrolysis-high performance liquid chromatography |
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| SUN Lizhen,BIE Mei,FENG Feng,WANG Jun,ZHU Jianhua,LIU Yanming,ZHANG Feng.Detection of tryptophan in infant food by enzymatic hydrolysis-high performance liquid chromatography[J].China Dairy Industry,2019(9):47-51. |
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| Authors: | SUN Lizhen BIE Mei FENG Feng WANG Jun ZHU Jianhua LIU Yanming ZHANG Feng |
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| Affiliation: | (Shandong Institute for Food and Drug Control,Jinan, 250100,China;Shangdong Quality Control EngineeringTechnology Research Center Of Foods for Special Medical Purpose, Jinan,250100,China;Chinese Academy of Inspection and Quarantine, Beijing 100176, China) |
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| Abstract: | SUN Lizhen;BIE Mei;FENG Feng;WANG Jun;ZHU Jianhua;LIU Yanming;ZHANG Feng(Shandong Institute for Food and Drug Control,Jinan, 250100,China;Shangdong Quality Control EngineeringTechnology Research Center Of Foods for Special Medical Purpose, Jinan,250100,China;Chinese Academy of Inspection and Quarantine, Beijing 100176, China) |
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| Keywords: | High performance liquid chromatography Tryptophan Milk powder Midical food Enzymatic hydrolysis |
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