玉米皮阿拉伯糖阿魏酸酯体外抗氧化活性的研究 |
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引用本文: | 林奇龄,温其标,欧仕益,赖富饶.玉米皮阿拉伯糖阿魏酸酯体外抗氧化活性的研究[J].广州食品工业科技,2012(1):33-38,26. |
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作者姓名: | 林奇龄 温其标 欧仕益 赖富饶 |
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作者单位: | [1]华南理工大学轻工与食品学院,广东广州510641 [2]暨南大学食品科学与工程系,广东广州510632 |
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摘 要: | 本文采用稀盐酸水解玉米麸皮得到低聚糖阿魏酸酯(FOs),经D301大孔树脂的除杂和聚酰胺柱层析的分离获得FOs中的F-Ara组分,在分析结构后,对该组分进行了体外清除DPPH和超氧阴离子自由基的能力测定和还原力测定。结果表明,与标准物阿魏酸(FA)、BHA和维生素C相比,F-Ara均表现出良好的体外清除自由基活性,其活性随浓度的增加而增大;在试验浓度为0.6 mmol/L时,与标准物阿魏酸和维生素C相比,F-Ara显示出最高的还原值,说明F-Ara具有较强的还原力。以上结果揭示F-Ara可成为一种能应用到食品、药品和化妆品的天然高效的抗氧化剂。
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关 键 词: | 抗氧化活性 阿拉伯糖阿魏酸酯 自由基 玉米皮 |
In Vitro Antioxidant Activity of Feruloyl Arabinose from Maize Bran |
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Authors: | LIN Qi-ling WEN Qi-biao OU Shi-yi LAI Fu-rao |
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Affiliation: | 1 .College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China) (2.Department o f Food Science and Technology, Jinan University, Guangzhou 510632, China) |
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Abstract: | In this study feruloylated oligosaccharides (FOs) was released from maize bran by hydrochloric acid hydrolysis, and feruloyl ambinose (F-Ara) was obtained by D301 macroporous resin chromatography followed by polyamidc resin purification from FOs. After structural identification, the antioxidant activity ofF-Am was evaluated in vitro by DPPH and superoxide radical scavenging activity assay and reducing power assay. F-Ara exhibited a good radical-scavenging activity in vitro when compared to standard antioxidants such as ferulic acid (FA), BHA and L-ascorbic acid (VC). The radical-scavenging activity depended old the concentration and increases with increasing dose of sample. The highest reducing value of F-Ara compared with FA and VC at a dosage of 0.6 mmol/L indicated that F-Ara had a considerable reducing power. The present study suggested that F-Ara can be a natural and efficient antioxidant used in food, medicine and cosmetic. |
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Keywords: | antioxidant activity feruloyl arabinose free radical maize bran |
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