Identification of Cephalopod Species (Ommastrephidae and Loliginidae) in Seafood Products by Forensically Informative Nucleotide Sequencing (FINS) |
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| Authors: | M.J. Chapela,,C.G. Sotelo,,P. Calo-Mata,R.I. Pé rez-Martí n,H. Rehbein,,G.L. Hold,,J. Quinteiro,,M. Rey-Mé ndez,C. Rosa,,A.T. Santos |
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| Affiliation: | Authors Chapela, Sotelo, Calo-Mata, and Perez- Martin are with the Instituto de Investigaciones Marinas (CSIC), Eduardo Cabello 6, 36208 Vigo, Spain.;H. Rehbein is with the Institute of Fishery Technology and Fish Quality, Palmaille 9, 22769 Hamburg, Germany. G.L. Hold is with the Rowett Research Institute, Greenburn Road, Aberdeen AB21 9SB, Scotland, U.K.;J. Quinteiro and M. Rey-Mendez are with the Dept. de Bioquímica y Biología Molecular, Facultad de Biología, Univ. de Santiago de Compostela, Spain.;Authors Rosa and Santos are with the Instituto de Investigaçao das Pescas e do Mar, Av. Brasilia, 1400 Lisboa, Portugal. Direct inquiries to author Chapela Garrido (). |
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| Abstract: | ABSTRACT: An identification technique of commercially important cephalopods based on 16s RNA analysis was developed. A set of primers was designed to amplify a fragment of approximately 200 bp that presents enough variability for reliable species identification. Sequences from this fragment of 9 different authentic species were studied, genetic distances were measured, and a phylogenetic tree was constructed, with individuals of the same species grouped within the same cluster. Eight different types of commercial seafood products, mainly labelled as "squid rings", were analyzed and sequences were employed for species identification showing that FINS is a suitable technique for identification of processed cephalopods. |
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| Keywords: | seafood PCR species identification cephalopods 16s RNA gene FINS |
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