Spectral-domain phase microscopy with improved sensitivity using two-dimensional detector arrays |
| |
Authors: | Singh K Dion C Lesk M R Ozaki T Costantino S |
| |
Affiliation: | Centre de Recherche, H?pital Maisonneuve-Rosemont, Montre?al, Quebec, Canada. |
| |
Abstract: | In this work we demonstrate the use of two-dimensional detectors to improve the signal-to-noise ratio (SNR) and sensitivity in spectral-domain phase microscopy for subnanometer accuracy measurements. We show that an increase in SNR can be obtained, from 82 dB to 105 dB, using 150 pixel lines of a low-cost CCD camera as compared to a single line, to compute an averaged axial scan. In optimal mechanical conditions, phase stability as small as 92 μrad, corresponding to 6 pm displacement accuracy, could be obtained. We also experimentally demonstrate the benefit of spatial-averaging in terms of the reduction of signal fading due to an axially moving sample. The applications of the improved system are illustrated by imaging live cells in culture. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|