| 丙酮三重态对DNA定位损伤的直接鉴定 |
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| 引用本文： | 宋钦华,姚思德,林念芸.丙酮三重态对DNA定位损伤的直接鉴定[J].辐射研究与辐射工艺学报,1997(1). |
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| 作者姓名： | 宋钦华 姚思德 林念芸 |
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| 作者单位： | 中国科学院辐射化学开放研究实验室，上海原子核研究所!上海201800 |
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| 摘 要： | 利用纳秒级激光光解动态吸收光谱装置研究了多聚核苷酸及DNA水溶液中光敏化结果：首次观察到丙酮激发三重态分别与多聚鸟苷酸（PolyG]）、多聚腺－鸟苷酸（PolyA，G]）及单链DNA（ssDNA）反应生成鸟嘌呤抽氢自由基的瞬态吸收光谱，从而为丙酮三重态对DNA的定位损伤提供了直接证据。
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| 关 键 词： | DNA 激光光解 丙酮三重态 鸟嘌呤抽氢自由基 定位损伤 |
DIRECT CHARACTERIZATION SITE-SELECTIVE PHOTOSENSITIZED DAMAGE OF DNA BY TRIPLET ACETONE |
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| Song Qinhua, Yao Side, Lin Nianyun.DIRECT CHARACTERIZATION SITE-SELECTIVE PHOTOSENSITIZED DAMAGE OF DNA BY TRIPLET ACETONE[J].Journal of Radiation Research and Radiation Processing,1997(1). |
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| Authors: | Song Qinhua Yao Side Lin Nianyun |
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| Abstract: | The interaction of triplet acetone with polyguanylic acid(PolyG]).polyadenylic-guanylic acid(PolyA,G])and single-stranded(ssDNA) were investigated in neutral aqueous solution using KrF (248 nm) laser flash photolysis. The characterized absorption spectra of transient species were observed for the first time. On the basis of a comparison of the transient absorption spectra of the dehydrogenated radical of dGMP and those of PolyG], PolyA,G] or ssDNA, we suggest that the photosensitized transient species are guanyl radicals. Therefore, siteselective photosensitized damage of DNA at the guanine moiety was characterized. |
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| Keywords: | DNA Laser flash photolysis Triplet acetone Guanyl radical Site-selective damage |
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