| N-琥珀酰亚胺3 -[125I]碘苯甲酸酯(S125IB) 的放射化学合成 |
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| 引用本文： | 李俊玲,王丽华,张岚,田海滨,汪勇先.N-琥珀酰亚胺3 -[125I]碘苯甲酸酯(S125IB) 的放射化学合成[J].核技术,2003,26(3). |
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| 作者姓名： | 李俊玲 王丽华 张岚 田海滨 汪勇先 |
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| 作者单位： | 中国科学院上海原子核所放药中心,上海,201800 |
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| 摘 要： | 利用Iodogen法成功地对N-琥珀酰亚胺 3-(三正丁基锡)苯甲酸酯(ATE)进行了放射性碘-125标记,利用Sep-Pak硅胶柱实现了干扰蛋白标记的ATE及Iodogen与S125IB的分离,得到了放射性碘间标蛋白质有用的中间体S125IB,标记率93%以上。并研究了影响标记的因素,得出较佳标记条件为:ATE与 Na125I摩尔比6:1、氧化剂 Iodogen用量7mg、室温反应5min。
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| 关 键 词： | shiftasIodogen法 N-琥珀酰亚胺3-(三正丁基锡)苯甲酸酯 N-琥珀酰亚胺3-[125I]碘苯甲酸酯放射性碘标 |
Synthesis of radioiodinated N-Succinimidyl 3-[125I]Iodobenzoate |
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| LI Junling,WANG Lihua,ZHANG Lan,TIAN Haibin,WANG Yongxian.Synthesis of radioiodinated N-Succinimidyl 3-[125I]Iodobenzoate[J].Nuclear Techniques,2003,26(3). |
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| Authors: | LI Junling WANG Lihua ZHANG Lan TIAN Haibin WANG Yongxian |
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| Abstract: | N-Succinimidyl 3-(tri-n-butylstannyl)benzoate (ATE) was radioiodinated using Iodogen as oxidant and useful conjugate S125IB of labeling proteins was obtained . ATE and Iodogen affecting labeling proteins were successfully isolated from S125IB by Sep-Pak silica . The labeling efficiency was more than 93%. Several factors affecting labeling such as labeling time, the amount of Iodogen and the mole ratio of ATE to Na125I, were studied. The better labeling conditions were obtained as follows: mole ratio of ATE to Na125I = 6:1, Iodogen=7mg , labeling time = 5min in room temperature. |
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| Keywords: | Iodogen N-Succinimidyl 3-[125I]Iodobenzoate N-Succinimidyl 3-(tri-n-butylstannyl)benzoate Radioiodination |
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